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Isolation of the GAT1/GFP fusion protein from Sf9 cells by mAb-GFP-conjugated affinity column chromatography with analysis of the eluted fractions from the mAb-GFP-conjugated affinity column with 4 M MgCl2 (pH 6) by SDS-PAGE with silver staining and Western blotting. The arrow denotes the fragment of mAb-GFP. M: standard marker; CL: cell lysate; FT: flow-through; E1–E8: eluted fractions 1–8.
