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. 2017 May 11;13:874–882. doi: 10.3762/bjoc.13.88

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Copyright © 2017, Hu et al.

This is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)

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Purification of the GAT1/GFP fusion protein from Sf9 cells by SE-FPLC. (A) Elution profile of GAT1/GFP after SE-FPLC on a Superdex 200^TM column. (B) The standard linear regression curve of the Superdex 200^TM column was generated by plotting the log of the molecular masses of different calibration proteins against their elution volumes. (C), (D) Silver staining and Western blotting of the SDS-PAGE (7.5%) results of eluted fractions (from 8.8 to 11.8 mL) after SE-FPLC. M: standard marker; C: concentrated sample from immunoaffinity column; 1–10: SE-FPLC fractions from 8.8 to 10.8 mL.