Table 1.

Primers targeting cutC and cntA as well as the V1-2 region of the 16S rRNA gene (with modified forward primer) are shown

	Forward primer (5′➔3′)	Reverse primer (5′➔3′)
cutC	TTYGCIGGITAYCARCCNTT	TGNGGYTCIACRCAICCCAT
cntA	TAYCAYGCITGGRCITTYAARCT	RCAGTGRTARCAYTCSAKRTAGTTRTCRAC
16S	AGRGTTHGATYMTGGCTCAG	TGCTGCCTCCCGTAGGAGT

Bold letters highlight the degenerate bases within sequences (total combinations—cutC_F, 32; cutC_R, 16; cntA_F, 32; cntA_R, 512; 16S_F, 32; 16S_R, 1). Expected sizes of amplified products (excluding primers) are 275 bp (cutC) and 249 bp (cntA) based on references ACL49259 and EFK52065, respectively