Fig. 5.
Demonstration of bFGF role in maintaining stemness of human embryonic stem cell using chemical inhibitor (SU5402) of FGFR1: (A) Effect of inhibitor on secreted amount of TGF-β and IGF-II released by I-HFF in presence of bFGF. Results are mean±standard deviation of three independent experiments. **P<0.01 compared to bFGF alone; and (B) Phase contrast images of KIND-1 human embryonic stem cells showing visible differentiation (black arrow). The cells were cultured in conditioned medium from inhibitor-treated I-HFF.