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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Metab Eng. 2016 Oct 19;38:446–463. doi: 10.1016/j.ymben.2016.10.009

Figure 3. Purification, and quaternary structure analysis of HPCS and HPCD.

Figure 3

(A) HPCD was expressed in E. coli, purified by IMAC, and separated by size exclusion chromatography (Superdex 75). (B) Molecular assembly of HPCD as homooctomer. (C) HPCS and HPCD co-expressed in E. coli, purified by IMAC, and viewed on SDS-PAGE. (D) HPCS and HPCD separated by size exclusion chromatography (Superdex 75). (E) SDS-PAGE of two peaks eluted from size exclusion chromatography. (F) (HPCS)4(HPCD)8 determined by size exclusion chromatography.