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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Metab Eng. 2016 Oct 19;38:446–463. doi: 10.1016/j.ymben.2016.10.009

Figure 4. Purification and quaternary structure analysis of ACR and SSR.

Figure 4

(A) ACR and SSR were co-expressed in E. coli and recombinant enzymes were purified by IMAC. Quaternary structure was assayed by Superdex 75. (B) The two eluted peaks from (A) were collected and analyzed by SDS-PAGE. (C) NanoLC-MS/MS of the two peaks collected in (A). (D) SSR was expressed in E. coli and recombinant SSR purified by IMAC was analyzed by Superdex 75. (F) The collected peak of recombinant SSR in Superdex 75 was analyzed by SDS-PAGE.