Table 2.

Molecular properties of selected enzymes for 3HP to 4HB conversion

Enzyme	Substrate	Vmax (μmol min−1 mg−1)	Km (μM)	kcat/Km (105 M−1 s−1)	Mra (kDa)	Molecular assembly	Ref.
MCR	Succinyl-CoA	40b	150	1.75	39.3	NRd	(Kockelkorn and Fuchs, 2009)
		15.9	191	0.545		(MCR)3	This work
	NADPH	13.1	79.2	1.09
HPCS	3-Hydroxypropionate	18	180	1.24	74.4	(HPCS)4	(Alber et al., 2008)
HPCD	3-Hydroxypropionyl-CoA	272	25.1	51.2	28.3	(HPCD)8	This work
		151	60	11.9		(HPCD)1	(Teufel et al., 2009)
ACR	Acryloyl-CoA	7.6	9.1	5.01	36.0	(ACR)1	This work
		2.9c	NRd	NRd		NR	(Teufel et al., 2009)
		18.7b	10	11.2		(ACR)1	(Teufel et al., 2009)
SSR	Succinic semialdehyde	683	27	160	37.9	(SSR)5	This work
		700	70	63.2		(SSR)2	(Kockelkorn and Fuchs, 2009)

^aPredicted from sequence

^bRecombinant S. tokodaii, expressed in E. coli and purified

^cNative M. sedula, partially purified

^dNR, not reported