Skip to main content
. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Metab Eng. 2016 Oct 19;38:446–463. doi: 10.1016/j.ymben.2016.10.009

Table 4.

Overall stoichiometry for pathways constructed from 3HP/4HB cycle enzymes

Pathway Reaction ATP/CO2 NAD(P)H/CO2
Native cycle 3.3 CO2 + 8.6 ATP + 7.6 NADPH + 1.65 NAD → 3.3 Biomass (C1) + 8.6 ADP + 7.6 NADP + 1.65 NADH 2.6 1.8
Acetyl-CoA(autotrophic) 2 CO2 + 6 ATP + 5 NADPH + NAD + CoA → Acetyl-CoA + 6 ADP + 5 NADP + NADH 3.0 2.0
Succinate(autotrophic) 4 CO2 + 10 ATP + 9 NADPH + 2 NAD → Succinate + 10 ADP + 9 NADP + 2 NADH 2.5 1.8
Succinate(heterotrophic) Acetyl-CoA + 2 CO2 + 4 ATP + 4 NADPH + NAD → Succinate + 4 ADP + 4 NADP + NADH 2.0 1.5
3HP(heterotrophic) Acetyl-CoA + CO2 + ATP + 2 NADPH → 3HP + ADP + 2 NADP + CoA 1.0 2.0