Table 1.

Orthogonal array design matrix OAD OA₉(3)⁴ and experimental results of alkaloid 1 yield.

Run	Factors				Responses
	Cosolvent	t (min)	T (°C)	P (bar)	Y Ap ± σp (mg g−1) (n = 3)
1	Ethanol	20	40	150	0.29 ± 0.02
2	Ethanol	40	50	200	1.2 ± 0.3
3	Ethanol	60	60	250	1.59 ± 0.07
4	Methanol	20	50	250	1.0 ± 0.3
5	Methanol	40	60	150	0.7 ± 0.9
6	Methanol	60	40	200	3.8 ± 0.8
7	Propyleneglycol	20	60	200	1.9 ± 0.3
8	Propyleneglycol	40	40	250	1.86 ± 0.08
9	Propyleneglycol	60	50	150	0.73 ± 0.01

Y _Ap = yield of the alkaloid in the plant.

Results are expressed as mean ± standard deviation (σ).