Fig. 5. Age-dependent spatio-chemical differences in the hippocampus detected with TiO2-DA monolith-assisted LDI MSI. (A) Representative ion images of cholesterol (m/z 409.3). (B) PCA score plots of TiO2-DA monolith-assisted LDI MSI datasets acquired from different hippocampal and surrounding regions of young and old mice. (C) Statistical comparison of average peak areas of identified molecular signals (data points acquired from the same batch of old and young animal samples are marked by the same color). p values for datasets collected from animals of different ages; samples were calculated using two-way ANOVA, and related detailed information is provided in Table S5.† ***, p value < 0.001, **, p value < 0.01, *, p value < 0.05, +, p value < 0.1. CA1, region I of hippocampus proper; CA3, region III of hippocampus proper; DG, dentate gyrus; CC, corpus callosum.
