Figure 2.

This figure shows representative immunofluorescence staining and FACS analysis of outgrown cells cultivated with (a) EGM-2 and (b) SGM-2. In both settings, most cells expressed CD34 (red, upper row in both columns), whose portion decreased slightly during further cultivation. During cultivation with EGM-2, colony-forming cells positive for CD31 (green, middle row) and NOS (red, bottom both in the left column) became the predominant cell type. During cultivation with SGM-2, colony-forming LOSMC positive for sm-alpha-actin-positive (green, middle row) and about 57% positive for smoothelin (red, bottom row) were the predominant cell type. Compare Table 3 for antigen expression.