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. 2017 Mar 15;8:2041731417698852. doi: 10.1177/2041731417698852

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Cells which were cultivated with EGM-2 and taken from (a) not expanded cultures and (b) from the fourth passage, seeded on PTFE after 7 days of cultivation in a bioreactor (staining with calcein AM and ethidiumhomodimer-1, magnification 100×). Not expanded cells cultivated with EGM-2 formed a nearly coherent monolayer (a) in contrast to cells taken from the fourth passage (b). The percentage of the surface covered with LOEC and EC is shown in the both graphs (c under static conditions and d under dynamic conditions). (c) Under static conditions no significant differences were found between the different cell types. After perfusion in a bioreactor under dynamic conditions, coverage of the surface was significantly highest in setting with not expanded cells cultivated with EGM-2 in comparison to all other groups (p < 0.05), while no significant differences were found between the other settings.