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. 2017 May 16;12(5):e0177332. doi: 10.1371/journal.pone.0177332

Fig 3. Selective ROCK-2 inhibition by KD025 blocks rt-PA-induced effects on BBB permeability under stroke-like conditions.

Fig 3

(A) Permeability changes in the in vitro human BBB 7.5 h post stimulation under oxygen-glucose deprivation (OGD) with DMSO as control or with rt-PA (25nM) and human plasminogen (plgn; 100nM), with or without KD025 (0.2, 2 and 20μM, added to both luminal and abluminal chambers). Data is presented relative to DMSO control under normoxia. n = 3, Bars represent mean±SEM. *P<0.05, **P<0.01 compared to rt-PA+Plgn by one-way ANOVA with Newman–Keuls post hoc analysis. #P<0.05 compared to DMSO OGD control by two-tailed t-test. (B) Comparison of selective ROCK-2 inhibition by KD025 (20μM) versus non-selective ROCK inhibition by fasudil (HA1077; 20μM) against rt-PA+plasminogen (25nM+100nM, respectively) 6 h and 7.5 h after treatment under OGD (relative to DMSO control under OGD). n = 3 for 6 h, n = 4 for 7.5 h. Data points represent mean±SEM. *P<0.05 by one-way ANOVA with Tukey’s post hoc analysis. #P<0.05 compared to rt-PA+Plgn by one-tailed paired t-test.