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. 2017 May 16;12(5):e0177681. doi: 10.1371/journal.pone.0177681

Fig 1. Schematic of the inducible expression system used throughout this study.

Fig 1

Plasmids that were co-electroporated in E2.5 chicken embryos. A. The first plasmid is composed of a CAGGS ubiquitous promoter upstream of an mCherry red fluorescent protein fused to a nuclear localization signal (NLS), followed by an IRES element and an rtTa protein. B. The second construct is a bi-directional Tet-responsive element, flanked to one side by a green EGFP tagged with a CAAX box membranal signal. On the other side, a cDNA sequence (Rac1T17N or Cdc42 T17N) is fused to an HA tag. Both constructs are flanked by Tol2 sequences and electroporated along with a transposase plasmid (C) to ensure permanent genomic integration.