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. 2017 May 16;12(5):e0177871. doi: 10.1371/journal.pone.0177871

Fig 6. CDK9 regulates BIRC6 mRNA levels.

Fig 6

(A) Inhibition of CDK9 with dinaciclib, HY-16462, and flavopiridol significantly reduced BIRC6 and Mcl-1 mRNA levels in a dose-dependent manner. MYL-R cells were treated with dinaciclib (10 or 100 nM), HY-16462 (250 nM or 1 μM), and flavopiridol (250 nM or 1 μM) for 24 hours and BIRC6 and Mcl-1 mRNA measured by QRT-PCR. (B) Immunoblot analyses of lysates from the same conditions in (A) above had no substantial effect on BIRC6 protein. By contrast, Mcl-1 protein levels were reduced in a dose-dependent manner except with 10 nM dinaciclib that showed a substantial increase in Mcl-1 protein. (C and D) shRNA knockdown of CDK9 in MYL-R cells and immunoblot analysis recapitulated the data obtained in (B) above. The data presented here are representative of three independent experiments. * Represents p < 0.05.