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. 2017 May 16;13(5):e1006081. doi: 10.1371/journal.ppat.1006081

Fig 2. Multi-sequence alignment and comparison of the predicted galectin-related protein (GREP) sequences with the BS-90 and NMRI Biomphalaria glabrata variant GREP proteins.

Fig 2

Amino acid sequence predicted from an RNAseq assembly (RNAseq-GREP; KM975647) [18] is compared to 4 predicted proteins encoded within Scaffolds18083, 47310, 210741 and 5606. Near-complete ORFs for the GREP, encoding IgSF1/IgSF2 domains and galectin binding domain, were amplified from BS-90 (GREP1.1) and NMRI (GREP1.2) snail cDNA. Original peptide sequences (shaded grey) aligned with the translated scaffolds and the GREP1.1 and 1.2 proteins. Cysteines involved in the formation of Ig-loop domains are shown in boldface, while residues associated with the sugar-binding pocket of the galectin domain, as predicted by NCBI delta-blast, are shown as underlined bolded letters (Q/H, R, R, T/Q, N/G/S, W, A/S, V). Amino acid differences between GREP [18], GREP1.1 and GREP 1.2 are highlighted in blue.