Fig 3. PCR amplification of NMRI and BS-90 B. glabrata galectin-related protein (GREP) transcripts.

Complementary DNA synthesized from whole body RNA extracts of 10 individual NMRI and 10 BS-90 B. glabrata snails were used to generate amplification products of the near-complete coding region of the BS-90 GREP sequence. GREP amplicons for each snail sample (1–10) are shown. Primers to B. glabrata α-actinin served as a loading control. Note that GREP amplicons were generated using cDNA from all BS-90 samples tested, while only 4/10 NMRI snails produced amplicons, demonstrating differential GREP gene expression in the NMRI snail population.