Table 2. Other immune-related proteins from NMRI and BS-90 Biomphalaria glabrata plasma eluted from Schistosoma mansoni sporocyst membrane-enriched (Mem) and larval transformation protein (LTP) affinity columns.

Protein Identification	Scaffold No.	NMRI	BS90
PREDICTED: Lipopolysaccharide-binding protein-like [Biomphalaria glabrata] (100%); Sequence ID: XP_013070669.1	LGUN_random_Scaffold1023	$\mathbf{IVGNVAQFR}$ $\mathbf{ALDDGMNMVVIPQLNAVGDTGIALPVLK}$ $\mathbf{DLTFQNPR}$ $\mathbf{QIPLESDIADKFVIDYR}$	IVGNVAQFR $\mathbf{ALDDGMNMVVIPQLNAVGDTGIALPVLK}$ $\mathbf{DLTFQNPR}$ $\mathbf{LLSPVLFTDSYLETQHK}$
Thioester-containing protein (TEP) [Biomphalaria glabrata] (100%); Sequence ID: ACL00841.1	LGUN_random_Scaffold4524; LGUN_random_Scaffold10725; LGUN_random_Scaffold9700; LGUN_random_Scaffold8482; LGUN_random_Scaffold2300; LGUN_random_Scaffold76986; LGUN_random_Scaffold7766; LGUN_random_Scaffold45951; LGUN_random_Scaffold53075; LGUN_random_Scaffold6063; LGUN_random_Scaffold7260	FTPNIHIGR IIDLLNDGYQR NIAFTLPDSLVPESQR TYDYYEPANQATVFYQPR $\mathbf{HDTGMVVQELSIPSGFVPDLSTLGQVAGVK}$ $\mathbf{ITSTEAIDSLAYEIR}$ $\mathbf{QNIDGSFNEFGK}$ $\mathbf{SFFPESWLWTSVK}$ $\mathbf{SNMAADAIVR}$ $\mathbf{SQLAQSTFEK}$ $\mathbf{SYSMSNNYLQLSLLSK}$ $\mathbf{TETVSGAESQFFEVAEYDLPR}$ $\mathbf{VFRPFFVSLTYPR}$ $\mathbf{VGPSWAPIAQLLMYYIR}$ $\mathbf{DSTVCDVCPNCCP}$ $\mathbf{TLRDSTVCDVCPNCCP}$ $\mathbf{QNLDGSFNEFGK}$	IIDLLNDGYQR ITSTEAIDSLAYEIR NIAFTLPDSLVPESQR NIAFTLPDSLVPESHR SFFPESWLWTSVK TYDYYEPANQATVFYQPR $\mathbf{ATNQLSEELNKK}$ $\mathbf{FTPNIHIGR}$ $\mathbf{LDEALPSVR}$ $\mathbf{QNIDGSFNEFGK}$ $\mathbf{SQLAQSTFEK}$ $\mathbf{SYSMSNNYLQLSLLSK}$ $\mathbf{VFRPFFVSLTYPR}$ $\mathbf{VRSFFPESWLWTSVK\; ENPFLTPVTPGPGNQASNIQVR}$ $\mathbf{QNLDGSFNEFGK}$
PREDICTED: CD109 antigen-like [Biomphalaria glabrata] (100%) Sequence ID: XP_013071291.1	LGUN_random_Scaffold108	SVNQLSGDIQTK VTQPDGLPMTSTAEQVK $\mathbf{IYNVPVLIDLTEGK}$ $\mathbf{SVYFPIVPADLGK}$ $\mathbf{TTNTWDYPNPTK}$ $\mathbf{ALPVTTLQVPLNGEAK}$	$\mathbf{IYNVPVLIDLTEGK}$ $\mathbf{NQVTAFYQSQILK}$ $\mathbf{SVYFPIVPADLGK}$ $\mathbf{TVDLTLPSNTVK}$ $\mathbf{VTQPDGLPMTSTAEQVK}$
PREDICTED: Heat shock protein 70 kDa cognate 4 [Biomphalaria glabrata] (100%) Sequence ID: XP_013082115.1	LGUN_random_Scaffold253: BGLB007783	$\mathbf{ARFEELNADLFR}$ $\mathbf{IINEPTAAAIAYGLDK}$ $\mathbf{IINEPTAAAIAYGLDKK}$	$\mathbf{FEELNADLFR}$ $\mathbf{IINEPTAAAIAYGLDK}$ $\mathbf{IINEPTAAAIAYGLDKK}$ $\mathbf{LLQDFFNGK}$ $\mathbf{QTQTFTTYSDNQPGVLIQVYEGER}$
Heat shock protein 60 [Biomphalaria glabrata] (100%); Sequence ID: ACL00842	LGUN_random_Scaffold791: BGLB013300	$\mathbf{ALMLQGVDLLADAVAVTMGPK}$	$\mathbf{ALMLQGVDLLADAVAVTMGPK}$ $\mathbf{KPLLIVAEDVDGEALSTLVLNR}$
Biomphalysin [Biomphalaria glabrata] (100%); Sequence ID: AGG38744.1	LGUN_random_Scaffold10: BGLB000137	ASSPVTESIER FGDSSVPFYK FKGYLFNLESAR $\mathbf{ADGDDLYFLK}$ $\mathbf{ADGDDLYFLKK}$ $\mathbf{GYLFNLESAR}$ $\mathbf{NGFTWAADTR}$ $\mathbf{SVIEDLQAESVDSGVLYNR}$ $\mathbf{TNSGRPTFNYR}$ $\mathbf{TTVPYTAIITTK}$ $\mathbf{VGYFLQGLYR}$ $\mathbf{WEDGNGNFHQDYR}$	$\mathbf{FGDSSVPFYK}$ $\mathbf{FKGYLFNLESAR}$ $\mathbf{SVIEDLQAESVDSGVLYNR}$
Dermatopontin 2 [Biomphalaria glabrata] (100%); Sequence ID: AAZ80786	LGUN_random_Scaffold8114:1204:2972: BGLB013420	LTLVVPEGTAVK $\mathbf{CPDGQVVSYVSSIHNNRR}$ $\mathbf{FGFYCCDVQGSTPR}$ $\mathbf{QTHSCTDSGYVNDFDGPLVYTCPGNK}$ $\mathbf{WQFQICTL}$	$\mathbf{CPDGQVVSYVSSIHNNRR}$ $\mathbf{FGFYCCDVQGSTPR}$ $\mathbf{LTLVVPEGTAVK}$ $\mathbf{QTHSCTDSGYVNDFDGPLVYTCPGNK}$
PREDICTED: Millepora cytotoxin-1-like [Biomphalaria glabrata] (100%); Sequence ID: XP_013061974.1	LG24_random_Scaffold7889	FYCCTNKDYLVHACQHTLTINNAK GLFSSYDSTAGDR GLFSSYDSTAGDRLYR $\mathbf{VPEAMYVR}$ $\mathbf{GFISFRVPEAMYVR}$	GLFSSYDSTAGDR $\mathbf{GLFSSYDSTAGDRLYR}$
PREDICTED: Uncharacterized protein LOC106068784 [Biomphalaria glabrata] (100%); Sequence ID: XP_013083707.1 Uncharacterized protein containing a Zn-dependent metalloprotease ADAM/reprolysin-like subgroup domain	LGUN_random_Scaffold286	$\mathbf{SLPQIYNNLLPK}$ $\mathbf{AIGNLLGASNDGALSTSIMAIINAPGDVNR}$ $\mathbf{CSLGQCSSYSTPVVDTNCVFGDQK}$ $\mathbf{GNAVCGQIFCR}$ $\mathbf{IPNTMTCTPVYTSDGLVCDNQKR}$	$\mathbf{AIGNLLGASNDGALSTSIMAIINAPGDVNR}$ $\mathbf{SLPQIYNNLLPK}$ $\mathbf{CSLGQCSSYSTPVVDTNCVFGDQK}$
PREDICTED: A disintegrin and metalloproteinase (ADAM) with thrombospondin motifs [Biomphalaria glabrata] (100%); Sequence ID: XP_013075983.1	LGUN_random_Scaffold16032	$\mathbf{TYHTALLTAQQISELLGSQHDGVLSSITNR}$ $\mathbf{WFFSSAVAGDIK}$ $\mathbf{YAVSMSEFDR}$ $\mathbf{YAVSMSEFDRTYHTALLTAQQISELLGSQHDGVLSSITNR}$	No peptides
PREDICTED: Serpin B6-like [Biomphalaria glabrata] (100%); Sequence ID: XP_013077880.1	LGUN_random_Scaffold1827	IQSDVIDLVPALK SNLNQLLQNMK VDRPFLYVIR	No peptides
PREDICTED: 14-3-3 protein epsilon-like [Biomphalaria glabrata] (100%); Sequence ID: XP_013082420.1	LGUN_random_Scaffold260: BGLB007883	$\mathbf{DSTLIMQLLR}$	$\mathbf{AAFDDAIAELDTLSEESYK}$ $\mathbf{AAFDDAIAELDTLSEESYKDSTLIMQLLR}$ $\mathbf{DSTLIMQLLR}$ $\mathbf{YLAEFATGNDR}$
PREDICTED: Transforming growth factor-beta-induced protein [Biomphalaria glabrata] (100%); Sequence ID: XP_013079848.1	LGUN_random_Scaffold209	$\mathbf{FTTFYQLLK}$ $\mathbf{LPAGSLDALK}$ $\mathbf{LSAETLDYLNNHISDLTDVLR}$ $\mathbf{SDISTFESLLVK}$	$\mathbf{No\; peptides}$
PREDICTED: Neo-calmodulin-like [Biomphalaria glabrata] (100%); Sequence ID: XP_013067017.1	LG27_random_Scaffold161:BGLB001498	SLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMAR VFDKDGNGFISAAELR $\mathbf{EAFSLFDKDGDGTITTK}$	VFDKDGNGFISAAELR $\mathbf{EAFSLFDKDGDGTITTK}$
PREDICTED: Uncharacterized protein LOC106079481 [Biomphalaria glabrata] (100%); Sequence ID: XP_013096101 Ca-binding protein 1-like (100%); Sequence ID: AAV91525.1	LGUN_random_Scaffold6449	$\mathbf{ADYTAAIDENFPNETHDPIITNAYR}$ $\mathbf{FFQTYDANHDNR}$	$\mathbf{ADYTAAIDENFPNETHDPIITNAYR}$ $\mathbf{FFQTYDANHDNR}$
PREDICTED: GATA zinc finger domain-containing protein [Biomphalaria glabrata] (100%); Sequence ID: XP_013076718.1 Superoxide dismutase (Cu-Zn) [Megathura crenulata] (40%); XP_013083707.1, CAF22060.1	LGUN_random_Scaffold1689	DLTAKNEIPIFAR EGLLEKDLTAK HLNEQYKPNLK HNPIQIHIYKGEK NEIPIFAR QFLNLSSNDISLNK YNHIHSLNNQDLR YNHIHSLNNQDLRR $\mathbf{GCHSMGGHFNPEGVHHGYR}$ $\mathbf{HNPIQIHIYK}$ $\mathbf{MSVEQIKK}$ $\mathbf{NDDDNLQDHVHVHSK}$ $\mathbf{QLHGFHVHEFGDSSK}$ $\mathbf{QLNKNDDDNLQDHVHVHSK}$	DLTAKNEIPIFAR EGLLEKDLTAK HLNEQYKPNLK HNPIQIHIYKGEK NEIPIFAR QFLNLSSNDISLNK $\mathbf{HNPIQIHIYK}$ $\mathbf{MSVEQIKK}$ $\mathbf{QLHGFHVHEFGDSSK}$
PREDICTED: Uncharacterized protein LOC106059379 [Biomphalaria glabrata] (100%); Sequence ID: XP_013072448.1 Uncharacterized protein containing Cu-type II ascorbate-dependent monooxygenase, C-terminal domain	LGUN_random_Scaffold1196: 16772–16869; 15959–16119; 14884–15048; 11587–12745	QHVQILPGDEIITR SQSIPVDGCDWK $\mathbf{TFLSYGDLELIDMYCQLR}$	QHVQILPGDEIITR $\mathbf{GLVSTIR}$ $\mathbf{SQSIPVDGCDWK}$

Protein identifications (Sequence ID) were determined using Mascot and Sequest against the current Biomphalaria glabrata annotated NCBInr database. Letters highlighted in light gray = peptides recovered from S. mansoni Mem affinity columns; Letters highlighted in dark gray = peptides recovered from LTP affinity columns. Non-highlighted black lettering = peptides common to both Mem and LTP columns. See S5 and S6 Tables for predicted protein sequences and locations of coding regions within the B. glabrata assemblage (VectorBase) for each identified protein.