Table 3. Intra- and inter-day precision and accuracy of quality control samples for targeted TCA metabolites.

TCA metabolite (Nominal concentration)	Intra Quality Control Levels (Nominal Conc. (μM))
	LLOQ QC (0.5 μM)			LQC (7.5 μM)			MQC (20.0 μM)			HQC (40.0 μM)
	A	%CV	% Accuracy	A	%CV	% Accuracy	A	%CV	% Accuracy	A	%CV	% Accuracy
Pyruvic acid	0.52	5.54	105	7.91	4.85	106	21.6	8.23	108	44.4	8.54	111
Citric acid	0.48	7.71	95.7	7.57	6.35	101	19.8	2.68	99.2	42.0	7.04	105
Cis-aconitic acid	0.51	10.6	103	6.96	6.06	92.8	19.1	7.52	95.6	40.4	8.50	101
2-Oxoglutaric acid	0.52	8.59	104	7.07	8.03	94.3	19.7	9.46	98.7	39.4	6.10	98.5
Succinic acid	0.52	10.2	105	6.64	6.57	88.6	18.5	2.00	92.5	42.1	2.76	105
Fumaric acid	0.48	8.46	98.8	6.70	3.91	89.3	19.0	2.75	94.9	36.6	2.58	91.5
Malic acid	0.54	5.02	107	7.09	9.41	94.5	18.3	2.22	91.6	41.5	2.77	104
Oxaloacetic acid	0.52	8.79	104	7.23	7.19	96.5	19.4	5.24	97.2	38.1	3.22	95.2
TCA metabolite (Nominal concentration)	Inter Quality Control Levels (Nominal Conc. (μM))
	LLOQ QC (0.5 μM)			LQC (7.5 μM)			MQC (20.0 μM)			HQC (40.0 μM)
	A	%CV	% Accuracy	A	%CV	% Accuracy	A	%CV	% Accuracy	A	%CV	% Accuracy
Pyruvic acid	0.52	8.36	105	7.46	6.13	99.5	20.1	8.22	100	40.1	8.82	100
Citric acid	0.49	7.56	99.0	7.24	7.79	97.4	19.0	6.06	95.0	38.5	5.71	96.2
Cis-aconitic acid	0.53	8.48	106	7.03	7.52	93.7	18.9	8.38	94.6	38.1	6.25	95.2
2-Oxoglutaric acid	0.49	7.45	98.8	7.11	9.32	94.9	20.6	5.25	103	41.3	5.52	103
Succinic acid	0.48	8.75	96.4	6.83	8.12	91.1	19.7	8.27	98.7	39.7	5.06	99.3
Fumaric acid	0.54	6.51	107	6.80	7.17	90.7	19.1	6.29	95.5	37.7	3.85	94.2
Malic acid	0.53	7.94	106	7.45	5.70	99.4	18.3	3.72	91.5	38.3	4.15	95.3
Oxaloacetic acid	0.52	6.42	103	7.75	6.09	103	19.8	6.11	98.8	38.8	3.17	97.0

A: Mean concentration (μM), %CV: Percent co-efficient of variance, LLOQ QC: Lower limit of quantitation quality control

LQC: Low quality control, MQC: Middle quality control, HQC: High quality control.