Fig 5. Expression and intracellular localization of G3GFP and TagRFP fused with peroxisome- or plastid-targeting signals in transformed A. thaliana with different combinations of promoters.

(A, B) Structural diagram of the binary constructs with peroxisome-targeting signals (constructs 2 and 7). (C, D) Fluorescent images of the leaf epidermis of A. thaliana transformed with construct 2 (C) or 7 (D). (E, F) Structural diagram of the binary constructs with plastid-targeting signals (constructs 3 and 8). (G, H) Fluorescent images of the leaf epidermis of A. thaliana transformed with construct 3 (G) or 8 (H). Different developmental stages in stomatal lineages are indicated by arrows (meristemoids), stars (GMCs), asterisks (immature GCs), or arrowheads (mature GCs). In meristemoids, only the expression of the fluorescent protein directed by P_MUTE was observed, while both signals were detected in later stages. GFP, signal of G3GFP; RFP, signal of TagRFP; Overlay, overlay of GFP and RFP; DIC Merged, differential interference contrast (DIC) merged with GFP and RFP; Boxed Area, enlargement of the boxed area in the overlay. Scale bars = 10 μm.