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. 2017 May 4;13(5):e1006359. doi: 10.1371/journal.ppat.1006359

Fig 1. Insertion of genetic barcode into SIVmac239.

Fig 1

(A) A 34 base cassette (yellow) bearing a stretch of 10 random bases was inserted between vpx and vpr of wild-type SIVmac239 to generate the genetically barcoded virus SIVmac239M. The MluI restriction site used is outlined in black, and the sequences of the barcode flanking regions are colored in blue and green to depict the two possible insertion orientations. (B) Representative sequences in single stock aliquots depicting the bimodal distribution of authentic barcodes (green) versus barcodes containing PCR error (red).