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. Author manuscript; available in PMC: 2017 May 16.
Published in final edited form as: Virus Res. 2017 Feb 17;232:123–133. doi: 10.1016/j.virusres.2017.02.007

Fig. 1.

Fig. 1

(a) A schematic of the RRV genome indicating the position of the five ORFs above which the locations of the 1st and 2nd round amplicons are shown. The PCR primers used are indicated at the ends of each amplicon while the size of each product is given in Kb below. (b) Gel analysis of representative purified RT-PCR products generated for sequencing. Products generated from three RRV samples are shown; NY.1990.1924 generated 1st round amplicons (A, B, C) for all targets; VT.2007.0294 generated 1st round amplicons for targets B and C but required 2nd round PCR to generate the A1 and A2 products; VT2005.0277 required 2nd round PCR for all three targets to generate six products (A1, A2, B1, B2, C1, C2). A 1Kb size marker run in parallel with all products confirms their expected sizes.