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. 2017 May 2;6:e25366. doi: 10.7554/eLife.25366

Video 11. Mitotic progression of CENP-C depleted Drosophila S2 cells expressing EGFP-Mps1K231A/F234A and mCherry-α-Tubulin (related to Figure 5C,D).

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DOI: 10.7554/eLife.25366.032

S2 cells depleted of CENP-C expressing mCherry-α-Tubulin (red) and EGFP-Mps1K231A/F234A (green) monitored by spinning disk confocal microscopy. Video illustrates that impairing the interaction between Mps1 and PP1-87B delays mitotic exit in kinetochore-null cells. Each frame represents a maximal intensity projection acquired every 4 min. NEB corresponds to time 0:0. Time is shown minutes.

DOI: http://dx.doi.org/10.7554/eLife.25366.032