Video 12. Mitotic progression of Drosophila S2 cells co-depleted of CENP-C and BubR1 expressing EGFP-Mps1^K231A/F234A and mCherry-α-Tubulin (related to Figure 5C,D).

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DOI: 10.7554/eLife.25366.033

S2 cells co-depleted of CENP-C and BubR1 expressing mCherry-α-Tubulin (red) and EGFP-Mps1^K231A/F234A (green) monitored by spinning disk confocal microscopy. Video illustrates that the depletion of BubR1 from kinetochore-null cells expressing EGFP-Mps1^K231A/F234A accelerates anaphase onset. Each frame represents a maximal intensity projection acquired every 4 min. NEB corresponds to time 0:0. Time is shown minutes.

DOI: http://dx.doi.org/10.7554/eLife.25366.033