Video 4. Inducible speckle imaging (ISI) in a metaphase spindle of a control S2 cell expressing GFP-α-Tubulin and CID-mCherry (related to Figure 1H,I and Figure 1—figure supplement 2).
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DOI: 10.7554/eLife.25366.009
The Video illustrates ISI imprinting and progressive contrast fade-out of GFP–α-tubulin in a control metaphase spindle. The observed speckle contrast fadeout of tubulin is due to microtubule turnover. The time between frames is variable and is presented on the corresponding excel source data file.