Video 5. Inducible speckle imaging (ISI) in a metaphase spindle of a PP1-87B depleted S2 cell expressing GFP-α-Tubulin and CID-mCherry (related to Figure 1H,I and Figure 1—figure supplement 2).
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DOI: 10.7554/eLife.25366.010
The Video illustrates ISI imprinting and progressive contrast fade-out of GFP–α-tubulin in a PP1-87B depleted metaphase spindle. The observed speckle contrast fadeout of tubulin is due to microtubule turnover. The time between frames is variable and is presented on the corresponding excel source data file.