(A–B) In vivo imaging of the chemiluminescent compounds luminol and lucigenin, showing myeloperoxidase activity (A) or ROS production (B) in the injured ears of Mus (red bars) and Acomys (blue bars). Images below graphs are representative for each timepoint. Chemiluminescence is measured in radiance [photons (p) per second (s) emitted from a square centimeter of tissue (cm2) and radiating into a solid angle of one steradian (sr)]. For luminol experiments: n = 7 Mus (14 ears) and n = 6 Acomys (12 ears) repeated measures ANOVA, F = 5.095, p<0.001 and for lucigenin experiments: n = 8 Mus (16 ears) and n = 6 Acomys, (12 ears), repeated measures ANOVA F = 4.536, p<0.001, *p<0.05 Sidak's multiple comparison test between species at the time indicated.
DOI:
http://dx.doi.org/10.7554/eLife.24623.009