Figure 7. Activation of P1 neurons suppresses male sleep and experimental activation of FRU neurons elicits calcium responses in MS1 neurons.
(A) Sleep profile of males expressing NaChBac in MS1 neurons (MS1>NaChBac) and control males (MS1/+ and NaChBac/+) in an iso31 control (left), fruLexA/4-40 mutant (middle) and dsx683/1649 mutant (right) background. N = 11–83. (B) Sleep profile of male (M) and female (F) flies in which P1-split-Gal4 (Inagaki et al., 2014) was used to drive UAS-TrpA1 (P1 > TrpA1) and parental control flies (P1/+ and TrpA1/+). TrpA1 was activated on the 2nd day by raising the temperature from 22°C to 29°C. N = 82–91. (C) GCaMP6m response to ATP (△F) in MS1 neurons of a male brain in which FRU neurons are activated by P2X2 expression and ATP perfusion. (D) Normalized GCaMP6m response (△F/F0) in the cell bodies of MS1 neurons in males (M) and females (F). N = 5–7. One-way ANOVA followed by Dunnett post hoc tests (A, B); Student’s t test (C, D).