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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Invest Dermatol. 2016 Dec 22;137(5):1042–1050. doi: 10.1016/j.jid.2016.11.037

Figure 1. Age-independent accumulation of CD8+CD28− T cells in the blood and skin of SSc patients correlates with the extent of skin fibrosis.

Figure 1

Representative CD28 expression profiles by peripheral blood CD8+ T cells in controls or patients (a). Lymphocyte population was gated according to light scatter characteristics (FSC/SSC). CD8+ T cells were identified as CD8+CD3+ cells. Proportion of circulating CD8+CD28− T cells in patients (n=65) and controls (n=32) (b) or in dcSSc (n=28) and lcSSc (n=37) patients (c). Groups in (b) and (c) are shown as boxplots with the median indicated as horizontal line and whiskers from minimum to maximum. P values were determined by Wilcoxon rank sum test. (d) Representative cell surface phenotype of skin-resident CD8+ T cells in normal (NS, n=5) or SSc (n=5) skin. Freshly isolated skin cell suspensions were gated on lymphocyte scatter and CD8 positivity. Comparison of skin-homing receptor expression (E) and skin-resident marker expression (f) by CD8+CD28− T cells in blood and skin of SSc patients. Statistics by ANOVA followed by post hoc Tukey’s test.