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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Invest Dermatol. 2016 Dec 22;137(5):1042–1050. doi: 10.1016/j.jid.2016.11.037

Figure 2. Blood and skin-resident SSc CD8+CD28− T cells exhibit effector and effector/memory phenotypes.

Figure 2

(a–b) Cell surface CD28 expression by circulating CD8+ T-cell subsets. (a) Data are gated on lymphocyte scatter and CD8 positivity. CD8+-gated cells were separated into naïve (CD45RA+CD27+), CM (CM, CD45RA−CD27+), effector/memory (EM, CD45RA− CD27−), and effector (E, CD45RA+CD27−) based on CD45RA and CD27 expression. Each of these subsets was analyzed for CD28 co-expression. A representative example is depicted. (b) Frequency of CD8+CD28− T cells in total, effector, naïve, CM and EM CD8+ T cells from SSc patients (n=29) and NDs (n=18). Each symbol represents one patient or a control. The mean response is shown as a horizontal line. (c) Expression of CD45RA and CD27 by SSc skin-resident CD8+CD28− T cells. CD8+CD28− T cells were gated as described in Figure 1d. A representative example is shown (upper panel). Phenotype of skin-resident CD8+CD28− T cells from 5 patients. In panels (b and d), statistics by ANOVA followed by post-hoc Tukey’s test.