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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Physiol Behav. 2017 Feb 14;176:40–49. doi: 10.1016/j.physbeh.2017.02.011

Figure 4.

Figure 4

Chronic GHSR reduction in the LHA does not affect energy balance in female rats. Body weight gain (A) and chow intake (B) were not affected by the LHA-targeted Ghsr knockdown mediated by the Ghsr AAV injection. Similarly after HFHS diet challenge the body weight (C), chow (D), lard (E), and 30% sucrose water (F) intake were not altered by the knockdown. Fat mass in brown, gonadal and inguinal adipose tissues (BAT, GWAT and IWAT respectively; G) was also not altered. Seven weeks after AAV construct injections Ghsr mRNA expression was reduced by ∼25% in the LHA of rats treated with Ghsr AAV compared with controls (H). Representative AAV construct injection location (I). ARC: arcuate nucleus; VMH: ventromedial hypothalamus. Blue represents the DAPI nuclear stain. Timeline provides the timing of PR tests and maintenance diet changes relative to AAV infusions. Data are expressed as mean ±SEM. n=12 (female rats) per treatment group.