Figure 1. SAMP × NOD2−/− mice show decreased severity of inflammation compared to SAMP WT mice.
(A) PCR genotyping of mouse tail DNA showing the generation of SAMP × NOD2−/− mice using speed congenic techniques. (B) Ileal total inflammatory scores at different time points (4, 10 and 20-30-wk old), as determined by the sum of chronic inflammation, active inflammation, villous distortion and mononuclear inflammation. ** P < 0.001 (n=17-24), unpaired Student’s t test; data are represented as mean ± SEM. (C) Representative histopathological sections show significant villous blunting and a significant increase of inflammatory cells in the lamina propria of 20-30-wk old SAMP WT compared to SAMP × NOD2−/− mice. (D) MPO activity in ilea of SAMP WT and SAMP × NOD2−/− mice at 30 wks of age. * P < 0.05 (n=13), pairwise Mann–Whitney test; data are represented as mean ± SEM.