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. 2017 Apr 15;50(2):63–69. doi: 10.1267/ahc.17002

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2017 The Japan Society of Histochemistry and Cytochemistry

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — In situ hybridization of Notch2 and immunohistochemistry of SOX2 in a sagittal section of rat pituitary gland at embryonic day 14.5. a) Hematoxylin and eosin staining. b) In situ hybridization of Notch2. c) Negative control of in situ hybridization using a sense probe of Notch2. d) Double staining of Notch2 detected by in situ hybridization and SOX2 detected by immunohistochemistry. Immunoreactivity for SOX2 was revealed by 3,3'-diaminobenzidine (brown) and the Notch2 in situ hybridization signal with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (blue). Arrow: prospective pars intermedia, Double arrow: prospective pars distalis, Triple arrow: prospective pars tuberalis. Bar = 100 μm.