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. 2017 Apr 22;50(2):85–93. doi: 10.1267/ahc.17011

Fig. 4.

Fig. 4.

Expression of the ERα/α homodimer induced by estradiol in MCF-7 cells and time comparison. (A) Detection of ERα homodimers using N-SIM. Cells were double-stained for anti-ERα antibody clones 6F11 (Alexa Fluor 594: red) and SP-1 (Alexa Fluor 488: green). Homodimers were represented by the yellow areas, and the nuclei labeled blue (DAPI). Bar = 5 μm. (B) The ratios of ERα/α homodimers were quantified as the yellow areas in the nuclei using Lumina Vision. *p = 0.0121 vs. control for the ERα/α homodimer. (C) Immunoblotting analysis of ERα proteins in breast carcinoma cells. β-actin functions as a control. (D) Detection of ERα homodimers using PLA. Homodimers were represented by the red dots (Texas red), and nuclei stained blue (DAPI). Bar = 50 μm. (E) The number of ERα/α homodimers was quantified as the area of the dots in the nuclei using Lumina Vision. *p = 0.0252 vs. control for the ERα/α homodimer.