Fig. 5.

The expression of TH-, CR- and CB-immunopositive neurons in lateral, middle and medial sections. TH-, CR- and CB-expressing neurons in medial (med), middle (mid) and lateral (lat) sagittal sections of the MOB in wild-type (WT) (A–I’, S, T, U) and Pkr2^−/− mice (KO) (J–R’, V, W, X) (for the definitions of med, mid and lat sections, see Materials and Methods). The areas surrounded by rectangles in A to R are shown in A’ to R’ with higher magnification, respectively. The rectangles in the A’ to I’ covered the area from the surface to the inward edge of the GL. On the other hand, the rectangles in the J’ to R’ covered the area from the outer edge to the inner edge of the outermost cell-dense region. The number of TH-, CR- and CB-expressing neurons in the GL or outermost cell-dense region of the MOB of WT and Pkr2^−/− mice in the rectangles (A’–R’) were counted and cell density of med, mid, lat and total of the three sections were calculated per 40,000 μm² (Y). In Pkr2^−/−, TH-immunopositive neurons were dramatically decreased in number in all sections we counted (Y). In contrast, CR- and CB-immunopositive neurons did not show a decrease in number, but showed an increase in number in Pkr2^−/− (Y). Bars = 500 μm (A–R); 100 μm (A’–R’); 50 μm (S–X).