Figure 4. NMR based evaluation of binding of compounds 4014008 and 7933989 to P32 protein.

The 1D ¹H-aliph spectra of 5 μM P32 in the absence (black) and presence of compounds #4014008 (A) and #7933989 (B) (red for 100 μM and blue for 500 μM) were collected. As a control, the spectrum of p32 in the presence of the known peptide binder, TT1 at 70 μM, was also collected (green). In presence of both compounds there is a shift in the peak at around −0.3 ppm (dashed line) and the concomitant appearance of a shoulder peak (indicated by arrows) similar to what observed in presence of the reference peptide TT1.