Skip to main content
. 2005 Jan;25(2):545–553. doi: 10.1128/MCB.25.2.545-553.2005

FIG. 4.

FIG. 4.

MTBP induces an increase in the amount of ubiquitinated p53 and a decrease in the ubiquitination of MDM2. (A) H1299 cells were cotransfected as indicated. Total cell lysates were analyzed by Western blotting as indicated. (B) H1299 cells were cotransfected as in panel A. Cellular extracts were immunoprecipitated (IP) with antiubiquitin P4D1 or isotype control antibody (data not shown) followed by Western analysis (W) with an anti-p53 antibody (Ab2433). (C) H1299 cells were cotransfected as in panel A, immunoprecipitated as in panel B, and Western blotted with an anti-MDM2 antibody (Ab-1). (D) Cell extracts prepared as for panels B and C were immunoprecipitated with anti-MDM2 SMP-14 followed by Western blotting with an anti-MDM2 antibody (Ab-1).