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. 2017 May 16;7:1966. doi: 10.1038/s41598-017-01982-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multidimensional scaling (MDS) plot of the logarithm of fold-change (logFC) values for the most variable genes in the 35 assembled transcriptomes of Rhinella spinulosa. Symbols for El Tatio, Chile are shown in blue and Catarpe, Chile in orange. Circles correspond to treatment at 20 °C, and triangles to treatment at 25 °C; while the filled circles/triangles correspond to Gosner stage 36 and open circles/triangles to Gosner stage 42.