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. 2017 May 16;7:1987. doi: 10.1038/s41598-017-02260-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Administration of Bc increases CD4⁺Foxp3⁺ Tregs for modulating ST-induced T cell responses. The proportion of Th1 (A), Th2 (B) and Foxp3⁺ Treg (C) populations in spleen of the controls or probiotic-treated mice were analyzed by FACS. IL-4, IL-5 and IL-13 production (D) was evaluated by ELISA in culture supernatants. Levels of Foxp3, TGF-β and IL-10 gene expression (E) were analyzed by real-time PCR. Relative expression was calculated using the 2^−ΔΔCt method after normalizing to the housekeeping transcript hprt. Results are expressed as means ± SD. Statistical significance (p < 0.05) was marked by different letters in T cell subset analysis. *p < 0.05, **p < 0.01, ***p < 0.001.