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. 2005 Jan;25(2):661–670. doi: 10.1128/MCB.25.2.661-670.2005

FIG. 2.

FIG. 2.

p53-dependent transcription in H2AX+/+ and H2AX−/− MEFs (A) and thymocytes (B) after DNA DSB damage. The mRNA levels of each gene were determined by quantitative real-time PCR and standardized by the mRNA levels of GAPDH as described previously (13). The mRNA levels in the untreated H2AX+/+ cells are arbitrarily set to 1. The MEFs were harvested 12 h after doxorubicin (Dox) treatment (0.5 μM), and the thymocytes were harvested 4 h after treatment with 5 Gy of IR. Mean values from three independent experiments are presented, with error bars indicating standard deviations.