Figure 2.

T and B cell frequencies in lungs following immunization. Mice were immunized twice with a mix of Hirep1, CTH93, and CAF01 with 2-week intervals using either the intranasal (IN), subcutaneous (SC), or simultaneous (SIM) immunization strategy. Two weeks after the last immunization, B cell- and T cell-mediated immune responses were depicted. (A,B) Frequencies of anti-CD45.2^{−(in vivo)} anti-B220⁺, and anti-IgA⁺ B cells were assessed by flow cytometry (FACS) on pools of lung cells (n = 8). Pools of lung cells (n = 8) were restimulated in vitro with a mixture of Hirep1 and CTH93 and frequencies of interferon gamma (IFNγ) and interleukin-17-producing antigen (Ag)-specific T cells (CD4⁺CD44^high and anti-CD45.2^{−(in vivo)}) were assessed by intracellular flowcytometry (C,D) or frequencies of IFNγ-producing Ag-specific T cells (CD4⁺CD44^high) are depicted as FACS plot (E–G).