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. 2017 May 17;8:569. doi: 10.3389/fimmu.2017.00569

Figure 4.

Figure 4

Hirep1- and CTH93-specific antibody responses and IgA plasma cells in genital tract (GT) following vaginal Chlamydia trachomatis (C.t.) challenge. Mice were immunized twice with a mixture of Hirep1, CTH93, and CAF01 with 2-week intervals using either the subcutaneous (SC) or simultaneous (SIM) immunization strategy. A CAF01 adjuvant control was included. (A,B) Five weeks after the immunization protocol, mice were challenged vaginally with C.t., and vaginal swabs were collected on days 3, 7, and 10 postinfection (p.i.). Hirep1- and CTH93-specific IgG1 and IgA titers were determined in vaginal swabs by enzyme-linked immunosorbent assay (n = 12). A 10-fold vaginal swab dilution is depicted for IgG1 and 5-fold dilution for IgA. Dilutions represent the linear phase of the titration curves for all immunization groups. Graphs show mean ± SEM, and statistical analysis are done by Kruskal–Wallis (non-parametric)/Dunn’s test. (C) Seven days p.i., Hirep1- and CTH93-specific IgA ELISPOT was performed on GT cells from three to four pools of two to four mice. Graphs show mean ± SEM, and statistical analyses are done by Kruskal–Wallis (non-parametric)/Dunn’s test. **p < 0.01, ***p < 0.001, ****p < 0.0001.