TABLE 1.

Summary of kinetic data for wild-type and mutant FGF10-FGFR2b interactions

FGF10-FGFR2b	Parameter	Value
FGF10-FGFR2b	Kon (/M/s)a	1.38 × 105
	Koff (/s)a	8.58 × 10−2
	KD (M)b	6.23 × 10−7
E158A FGF10-FGFR2b	Kon (/M/s)	1.25 × 105
	Koff (/s)	7.57 × 10−2
	KD (M)	6.04 × 10−7
K195A FGF10-FGFR2b	Kon (/M/s)	1.22 × 105
	Koff (/s)	7.46 × 10−2
	KD (M)	6.13 × 10−7
E158A-K195A FGF10-FGFR2b	Kon (/M/s)	1.43 × 105
	Koff (/s)	8.69 × 10−2
	KD (M)	6.08 × 10−7
R78A FGF10-FGFR2b	Kon (/M/s)	2.24 × 104
	Koff (/s)	4.99 × 10−2
	KD (M)	2.23 × 10−6
A172F FGFR2b-FGF10	Kon (/M/s)	1.27 × 105
	Koff (/s)	7.88 × 10−2
	KD (M)	6.22 × 10−7

^aK_on and K_off were derived as described in Materials and Methods. χ² was <10% of R_max in all cases.

^bThe apparent affinity, K_D, is equal to K_off/K_on.