TABLE 2.

Summary of kinetic data of wild-type and mutant FGF10-heparin and wild-type and mutant FGFR2b-heparin interactions

Protein-heparin	Parameter	Value
FGF10	Kon (/M/s)a	6.33 × 104
	Koff (/s)a	2.27 × 10−2
	KD (M)b	4.38 × 10−7
E158A FGF10	Kon (/M/s)	6.36 × 104
	Koff (/s)	2.89 × 10−2
	KD (M)	4.55 × 10−7
K195A FGF10	Kon (/M/s)	5.13 × 104
	Koff (/s)	2.52 × 10−2
	KD (M)	4.90 × 10−7
E158A/K195A FGF10	Kon (/M/s)	6.71 × 104
	Koff (/s)	2.86 × 10−2
	KD (M)	4.22 × 10−7
FGFR2b	Kon (/M/s)	6.85 × 104
	Koff (/s)	1.58 × 10−2
	KD (M)	2.31 × 10−7
A172F FGFR2b	Kon (/M/s)	6.67 × 104
	Koff (/s)	1.65 × 10−2
	KD (M)	2.48 × 10−7

^aK_on and K_off were derived as described in Materials and Methods. χ² was <10% of R_max in all cases.

^bThe apparent affinity, K_D, is equal to K_off/K_on.