Figure 2.

HOP/STI1 and Hsp90 are both expressed by SVZ migratory neuroblasts. (A) Migratory cells originating from SVZ explants immunolabeled against anti-HOP/STI1 antibody reveal an ubiquitous expression pattern. (B) PSA-NCAM expression is used to characterize neuroblasts from the SVZ. (C) Double labeling indicates that HOP/STI1 expressing cells are neuroblasts. Inset shows an orthogonal section depicting a chain of HOP/STI1⁺/PSA-NCAM⁺ cells. (D) HOP/STI1 is expressed predominantly in the cellular processes in non-permeabilized SVZ cultures (arrowheads). Only few cells within the migratory chains exhibit perinuclear expression (arrow). (E) Analysis of PSA-NCAM expression characterizes these cells as neuronal precursors that co-express HOP/STI1, as can be seen in more detail in an orthogonal section (F, Inset). In (G), chains of migratory cells are also immunolabeled against anti Hsp90. In (H), Dapi nuclear staining demonstrates these cells organized as chains, which is a hallmark of SVZ neuroblasts. Nuclear staining and Hsp90 expression are both depicted in the merge in (I). Scale bars: (A,B) 20 μm, (D,E) 20 μm, and (G–I) 10 μm.