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Expression of signaling proteins mediating insulin action in PTP1B−/− and PTP1B-reconstituted fibroblasts. Equal amounts of protein from PTP1B−/− and PTP1B-reconstituted (+PTP1B) fibroblasts were resolved by SDS-PAGE and immunoblotted with antibodies specific for PTP1B, TCPTP, IRβ, IRS-1, the p85 regulatory subunit of PI3K, the protein kinase Akt, the MAPK ERK2, and actin.
