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. 2005 Jan;25(2):612–620. doi: 10.1128/MCB.25.2.612-620.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 2. — Parafibromin (Parafib) is part of a human Paf1 complex. Antiparafibromin antibody Ab648 (lane 4), anti-hLeo1 antibody Ab677 (lane 6), and anti-hPaf1 antibody Ab674 (lane 8) immunoprecipitates from 293T cell lysates were immunoblotted with antiparafibromin antibody Ab648 (top panel), anti-hLeo1 antibody Ab677 (second panel), anti-hPaf1 antibody Ab673 (third panel), and anti-hCtr9 antibody Ab679 (bottom panel). Cell lysate was loaded in lane 1. Negative control (ctl) immunoprecipitations (IP) were performed with normal rabbit IgG (lane 2) and peptide-blocked controls (lanes 3, 5, and 7).