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. 2005 Jan;25(2):612–620. doi: 10.1128/MCB.25.2.612-620.2005

FIG. 5.

FIG. 5.

Characterization of parafibromin truncation mutant constructs. (A) Schematic diagram of parafibromin truncation mutant constructs and the L64P mutant construct representing germ line alterations identified from HPT-JT patients. (B) 293T cell lysates from cells transfected with Flag-tagged WT parafibromin or Flag-tagged mutant forms of parafibromin were immunoblotted with anti-Flag antibodies. (C) Immunoprecipitations (IP) with anti-Flag antibodies from 293T cell lysates cotransfected with either hLeo1 (left), hPaf1 (middle), or hCtr9 (right) together with the indicated Flag-tagged WT parafibromin or Flag-tagged parafibromin mutant constructs. Transfectants were immunoblotted with anti-hLeo1, anti-hPaf1, or anti-hCtr9 antibodies (top) or with anti-Flag antibody (bottom) as shown. Untransfected (UT) 293T cells were used as a negative control.