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. 2017 May 17;8:864. doi: 10.3389/fmicb.2017.00864

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Copyright © 2017 Cherifi, Jacques, Quessy and Fravalo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Composition of dead and live cells in the biofilm of Lm76 and Lm132 strains of L. monocytogenes formed in rich medium BHI and diluted medium BHI/10 for 24 h; (A) individual visualization of live population of the biofilm (left images), dead population (middle image) and the merge of the two images which represents the compilation of all images taken from the top of the biofilm to the bottom and corresponds to the total biomass formed in the biofilm (right image). (B) higher magnification (taken with a 100X objective) of Lm76 biofilm grown in the diluted medium BHI/10 showing dead (right image) and live (left image) biomass organization; arrows show filaments mostly present in dead biomass in the biofilm.