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. 2017 May 17;8:864. doi: 10.3389/fmicb.2017.00864

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Copyright © 2017 Cherifi, Jacques, Quessy and Fravalo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biofilm visualization of two strains of Listeria monocytogenes: Lm76 and Lm132 after 24 h of incubation at 30°C static conditions. Biofilm was grown in BHI medium (right images) and BHI/10 (left images); (A) Biofilm stained with Crystal violet 0.1% (B) 3D reconstruction, zoomed images of a view from above of L. monocytogenes biofilm stained with live/dead; Syto9 showing live cells in green and Propidium Iodide showing dead/damaged cells and e-DNA in red.